| Course ID: | GENE 8920. 3 hours. Repeatable for maximum 6 hours credit. |
| Course Title: | Nucleic Acids |
Course
Description: | Structure and function of nucleic acids. The isolation, structure, chemical analysis, hybridization, enzymology, and replication of DNA and RNA, nucleic acids enzymes, protein-nucleic acid interactions and recombinant DNA technology. |
| Oasis Title: | NUCLEIC ACIDS |
| Prerequisite: | BCMB 8020 or permission of department |
Semester Course
Offered: | Offered fall semester every year. |
| Grading System: | A-F (Traditional) |
|
| Course Objectives: | This course will provide an overview of nucleic acid structure and function. In
addition, students will learn current techniques for isolating and working with
both RNA and DNA. The proteins involved in carrying out both the synthesis and
degradation of nucleic acids will be discussed in detail. In addition, students
will be taught the latest recombinant DNA techniques. The basic biological
reactions involving DNA, i.e., replication, recombination and repair, will also
be taught. |
| Topical Outline: | 1. Historical overview
2. DNA structure and organization
3. DNA separation methods, chemical synthesis of DNA
4. Cloning DNA:Restriction enzymes/modification systems
5. Cloning DNA: Strategy, Vectors
6. DNA:DNA Hybridization, Southern transfers
7. Cloning DNA: DNA polymerases/reverse transcriptase
8. Cloning DNA: Ligases, polynucleotide kinase, terminal transferase
9. Cloning DNA: Library construction, recombinant selection and
preliminary characterization
10. Analysis of cloned DNA: DNA sequencing
11. Analysis of cloned DNA: DNA footprinting
12. Expression of cloned DNA
13. Alteration of cloned DNA: Nucleases and site-directed mutagenesis
14. Amplification of DNA: Polymerase chain reaction
15. Enzymatic reactions involving RNA: Nucleases
16. Enzymatic reactions involving RNA: poly(A) polymerases/
tRNA nucleotidyltransferases/capping and decapping
17. Enzymatic reactions involving RNA: Catalytic RNA/ribozymes
18. RNA structure and analysis: Isolation Procedures
19. S1 protection, Primer Extension Analysis
20. Riboprobes, Northerns
21. RT-PCR, RACE, Nuclear run-on Assays
22. Eukaryotic Transcription
23. Eukaryotic DNA Replication
24. DNA replication:termination in linear molecules/telomerases
25. Prokaryotic DNA replication: Initiation
26. Prokaryotic DNA replication: Replication fork movement
27. Prokaryotic DNA replication: Termination and stable DNA replication
28. DNA repair: excision repair
29. DNA repair: mismatch repair
30. DNA repair: postreplication repair
31. Genetic recombination: homologous
32. Genetic recombination:nonhomologous |
| Honor Code Reference: | All academic work must meet the standards contained in "A Culture of Honesty".
Each student is responsible to inform themselves about those standards before
performing any academic work in this class. Any infringements of the honor code
that come to the instructor’s attention will be remanded to the appropriated
UGA entity for investigation and disciplinary action. |