Course Description
Students will receive both classroom and laboratory training in the implementation of molecular techniques used for the diagnosis of avian diseases and the study of avian biology.
Athena Title
MOLECULAR AVIAN BIO
Prerequisite
BIOL 1107-1107L
Semester Course Offered
Offered fall
Grading System
A - F (Traditional)
Course Objectives
This laboratory course is intended to give students a solid foundation in state-of-the-art molecular techniques used to measure and manipulate DNA, RNA, and proteins and to provide hands-on-experience with these procedures as diagnostic tools to identify avian pathogens and as research tools in the study of avian biology. Students in this class will be expected to apply knowledge gained in the classroom setting through the designing, performing, and analysis of laboratory experiments.
Topical Outline
I. General molecular biology and lab technique II. Molecular sex determination of embryos III. Diagnosis and species determination of Coccidial infection IV. Mutational analysis of recessive white locus V. Sequence analysis of recessive white locus VI. Spatial mRNA expression analysis in the developing chick VII. Quantitative mRNA expression analysis in the chick VIII. Spatial protein expression analysis of the developing chick IX. Diagnosis of infectious bursal disease through protein detection X. Cell imaging through EGFP expression in chick embryos XI. Tissue specific regulation of EGFP expression in chick embryos XII. Avian transgenic technology
Syllabus