Course Description
The methods and strategies for genetic manipulation of prokaryotes--both bacteria and archaea--with an emphasis on primary literature. Classes will consist of discussions of both classic and current articles that describe the development of genetic methods for both well characterized and relatively uncharacterized microbes and the use of genetics as a tool to study cellular processes.
Athena Title
PROKARYOTIC GENE
Semester Course Offered
Offered spring
Grading System
A - F (Traditional)
Course Objectives
This course will teach genetic methods and their use for the study of cellular biology at a sophisticated/advanced level. This course will not be suitable for undergraduates and will offer students in departments like genetics, biochemistry, cell biology, and microbiology an advanced course in this subject.
Topical Outline
Course Outline: 1. Description of classic and current selection methods 2. Methods of DNA transfer (transformation, transduction, mating) 3. Strategies for selection of mutations in non-essential genes 4. Strategies for selection of mutations in essential genes 5. Proof of essentiality 6. Synthetic lethal selections 7. Characterization of phenotypes