Course Description
Basic understanding of commonly employed methods in experimental
therapeutics referencing the following topics: i) animal models
of stroke, diabetic retinopathy, cancer, pulmonary
hypertension/fibrosis, atherosclerosis, diabetic vascular
complications (I); and ii) cell culture models and molecular
biological techniques (II).
Athena Title
Method Experiment Therapeutics
Prerequisite
Permission of department
Semester Course Offered
Not offered on a regular basis.
Grading System
A - F (Traditional)
Student learning Outcomes
- Students will understand the basic experimental methods used in laboratory research.
- Students will develop logical ideas in handling the routine biological techniques.
- Students will develop a systematic approach toward research design and methods in experimental therapeutics
- Students will understand the importance of data analysis and interpretation in experimental therapeutics
- Students will develop the skills to identify potential pitfalls and suggest alternative research methods in experimental therapeutics
- Students will develop the skills to review literature for introducing new techniques in the ongoing research program
Topical Outline
- Methods of protein extraction and estimation
- Methods of protein detection I: Western blotting and immunoprecipitation
- Methods of protein detection II: Immunostaining and ELISA
- Cell proliferation and cell death studies and Oxidative stress: principles, detection, and measurements
- Basics of Cell Culture Methods
- Real-time qPCR and semi-quantitative RT-PCR, Primer designing and Cutting-edge Techniques (RNA silencing, gene editing, and Nano string technology)
- Molecular cloning, luciferase reporter assay, and flow cytometry
- Applications of liposomes and extracellular vesicles
- Cellular Bioenergetics and Metabolism using Seahorse Analyzer
Syllabus
Public CV
