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Instrumental Methods of Food Analysis


Course Description

Spectrophotometric, colorimetric, and chromatographic methods of analysis as applied to food. Emphasis will be placed on determining the most appropriate assay for the problem and then interpreting the results.

Additional Requirements for Graduate Students:
Graduate students will participate in teams to design a "virtual laboratory" appropriate for the analysis of food using computer technology. If only one student is enrolled in the course, then the graduate student will design, conduct, and prepare a report on a laboratory exercise.


Athena Title

Instrum Methods Food Analysis


Prerequisite

FDST 4040/6040-4040L/6040L


Semester Course Offered

Offered spring


Grading System

A - F (Traditional)


Student learning Outcomes

  • The students will understand fundamental physical and chemical principles that are involved in the analysis of food and food products.
  • The students will understand the process in developing good laboratory practices (GLPs)/techniques common to basic and applied research in food analysis.

Topical Outline

  • Review of organic/analytical chemistry basics
  • Introduction to food analysis
  • Evaluation of analytical data (precision/accuracy, reproducibility/repeatability)
  • Sampling and representative samples
  • Basic principles of spectroscopy, Electromagnetic spectrum, UV-vis, Fluorescence/chemiluminescence, Infrared (IR)
  • Atomic absorption and emission spectroscopies/ICP-A(O)ES Basic principles of chromatography, Adsorption chromatography, Partition chromatography, Ion exchange chromatography, Size exclusion chromatography, Affinity chromatography, Gas chromatography (GC), High performance liquid chromatography (HPLC)
  • Immunological assays (e.g., ELISA)
  • Electrophoresis
  • Nuclear magnetic resonance (NMR) spectroscopy, Mass spectrometry (MS)

Syllabus


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