Course Description
The separation, identification, and analysis of drugs and metabolites in biological samples.
Athena Title
Pharmaceutical Analysis II
Prerequisite
Permission of department
Semester Course Offered
Not offered on a regular basis.
Grading System
A - F (Traditional)
Course Objectives
a. To understand the importance of analysis of drugs and metabolites in biological samples b. To identify the special problems inherent in the types of biological samples used in bioanalytical problems c. To identify the chemical problems of drugs and metabolites that affect the assay methodology d. To define the advantages and disadvantages of the various analytical techniques of importance in bioanalytical work e. To apply the current analytical methodology to bioanalytical problems f. To understand the pitfalls and practical solutions to bioanalytical problems
Topical Outline
Chapter 1 Why Analyze Drugs in Biological Fluids Chapter 2 Special Problems with Biological Fluids Chapter 3 Special Problems in Structures of Drugs Chapter 4 Spectroscopy and Fluorimetry Chapter 5 Planar Chromatography Chapter 6 Gas Chromatography Chapter 7 High-Performance Liquid Chromatography Chapter 8 Saturation Analysis: Radioimmunoassay and Other Ligand Assays Chapter 9 Miscellaneous Methods of Analysis Chapter 10 Development and Evaluation Chapter 12 Pitfalls and Practical Solutions Notice: The course syllabus is a general plan for the course; deviations announced to the class by the instructor may be necessary. Principal course assignments: Each student is required to present a 20 - 30 minute class presentation on some aspect of chromatography with drug systems. Specific course requirements: There will be two(2) take-home exams worth 75% of the course grade. The remaining 25% of the grade is based on on the classroom presentation. Grading Policy: The standard A - F grading system will be followed. Attendance Policy: A student is expected to be present at each scheduled class period. The student must notify the instructor prior to the class period by e mail or telephone. Failure to notify the instructor will result in a drop of one letter grade in the final course. Text: The Analysis of Drugs in Biological Fluids, J Chamberlain, 2nd edition, CRC Press, Boca Raton, FL 1995 Policy for make-up examinations: Make-up exams will be allowed provided the student notifies the instructor prior to the exam period. The exam will be rescheduled soon after the illness or other outside event has concluded. Supplemental Text: Analytical Supercritical Fluid Chromatography and Extraction, edited by M Lee and K Markides, Chromatography Conferences Inc., Provo, UT 1990 Supplemental Text: High Performance Capillary Electrophoresis, Theory, Techniques and Applications, edited by MG Khaledi, Volume 146 in Chemical Analysis, Wiley-Interscience, 1998