Course Description
Multidisciplinary course for graduate students in the Life Sciences (biochemistry, cellular biology, genetics, and others) emphasizing the analysis of primary literature, didactic lecture content, contemporary experimental approaches, presentations, and projects that will provide a common set of core content and skills necessary for contemporary graduate studies emphasizing eukaryotic biology.
Athena Title
Adv Genetic Cell Biology BCMB
Semester Course Offered
Offered spring
Grading System
A - F (Traditional)
Course Objectives
Students will achieve: 1) understanding of current state of knowledge in the fundamentals of protein biochemistry and enzymology as the second semester of a 4 x 2 semester-hour course set that will cover the fundamentals of biochemistry,cellular biology, and genetics; 2) training in current approaches for investigation and elucidation of new knowledge in these areas; 3) initial preparation for success in developing an innovative research program involving experimental work and discovery in the field of biochemistry, cellular biology, or genetics. These outcomes will be achieved through lecture, discussion, projects, and use of primary research literature.
Topical Outline
Unit Lecture Biological content 4. Proteins 4.1 Amino acid chemistry, Proteomic analysis: Literature discussion: Amino acid chemistry 4.2 2°, 3°, 4° structure, Literature discussion: 2°, 3°, 4° structure 4.3 Motifs, domains, and modular elements of protein structure, Literature discussion: Motifs, domains, and modular elements of protein structure 4.4 Protein folding, roles of chaperones, Literature discussion: Protein folding, roles of chaperones, 4.5 Stabilization of protein structure, Literature discussion: Stabilization of protein structure 4.6 Protein expression, tagging, purification, preparation for biochemical characterization, Demo: Molecular structure visualization (Pymol project) 4.7 Methods for protein structure determination: crystallography, SAXS, Literature discussion: Methods for protein structure determination: crystallography, SAXS 4.8 Methods for protein structure determination: NMR, Literature discussion: Methods for protein structure determination 4.9 Protein Dynamics, modeling, docking, Literature discussion: Protein Dynamics, modeling, docking 5. Enzymology, metabolic control, metabolic disease 5.1 Protein-substrate interactions: kinetics and catalytic strategies, Literature discussion: Protein-substrate interactions: kinetics and catalytic strategies 5.2 Catalytic strategies and mechanisms: case studies: Literature discussion: Catalytic strategies and mechanisms: case studies 5.3 Coenzymes, cofactors and metals, Literature discussion: Coenzymes, cofactors and metals 5.4 Enzyme regulation and allosteric control, Literature discussion: Enzyme regulation and allosteric control 5.5 Enzyme regulation and allosteric control, Literature discussion: Enzyme regulation and allosteric control 5.6 Coordinate control of metabolism
Syllabus
Public CV