Course Description
Biochemical engineering concepts related to large-scale animal cell biotechnology and scalable manufacturing of cellular products, such as recombinant proteins, monoclonal antibodies, viral vaccines, therapeutic cells, and gene therapy vectors. Working in small groups, students will address a range of contemporary problems.
Additional Requirements for Graduate Students:
Graduate students are required to write a 2000-word bioethics
essay.
Athena Title
Animal Cell Biomanufacturing
Non-Traditional Format
The Cellular Biomanufacturing course will be taught through problem-based learning. The class will be given a problem statement and over the course of four weeks will be expected to formulate a problem resolution. At the end of each problem cycle, each group will submit a technical document and give a short presentation to the class.
Undergraduate Prerequisite
BCHE 3520
Graduate Prerequisite
Permission of department
Semester Course Offered
Offered every year.
Grading System
A - F (Traditional)
Course Objectives
1. Understand the benefits and limitations of animal cell culture 2. Apply mathematical models to cell growth and product synthesis 3. Identify, formulate, and solve problems related to the scale- up of biomanufacturing processes 4. Design a biomanufacturing system, component, or process to meet desired needs 5. Communicate effectively via a group setting about contemporary bio-manufacturing processes
Topical Outline
1. Introduction to Animal Cell Biotechnology 2. Effective technical communication 3. Library instruction 4. Primary vs. immortalized cell lines 5. Cell metabolism, proliferation, and death in culture 6. Microcarrier culture 7. Adaptation to serum-free suspension culture 8. Animal cell bioreactors: Complications and considerations 9. Genetic modification and stability 10. Modeling growth and substrate utilization 11. Scale-up vs. scale-out 12. In situ monitoring 13. Viral vaccine separation and purification 14. Product separation efficiency 15. Viral purification methods 16. Quality control 17. Regulatory aspects and GMP 18. Validation and product stability 19. Advances in biotechnology
Syllabus