Regenerative bioscience approaches to tissue repair and regeneration, including stem cell therapies, biomaterials, tissue engineering, gene therapies, and cell manufacturing approaches in regenerative medicine. Students will also learn about how novel devices, technologies, and diagnostic tools can enhance regenerative medicine strategies.
Athena Title
Therapies for Tissue Repair
Prerequisite
RBIO 2010 and (BIOL 1108 or BIOL 2108H)
Semester Course Offered
Offered spring
Grading System
A - F (Traditional)
Student learning Outcomes
Students will learn about regenerative bioscience approaches to tissue repair and regeneration.
Students will learn about endogenous repair processes and why the limitations of endogenous repair processes necessitate the development of regenerative medicine strategies.
Students will learn about biomaterials and tissue engineering, gene therapy, and bioreactor/cell manufacturing approaches in regenerative medicine.
Students will learn about how novel devices, technologies, and diagnostic tools can be used to enhance regenerative medicine strategies.
Topical Outline
I. Overview of Regenerative Bioscience
a. Brief History of Regenerative Bioscience
b. Mechanisms of Vertebrate Regeneration
i. Cellular Regrowth
ii. Regeneration from Pre-existing Parent Cells
iii. Transdifferentiation
iv. Activation of Adult Stem Cells
c. Modeling Approaches
II. Tissue Injury Responses
a. Inflammatory Cells
b. Pro- and Anti-inflammatory Cytokines and Chemokines
c. Inflammatory Cascade
d. Acute and Chronic Inflammation
e. Oxidative Stress
f. Mechanical Responses
III. Endogenous Repair Mechanisms
a. Skin
b. Neural Tissues
c. Musculoskeletal Tissues
d. Cardiac Tissue
e. Hemopoietic System
IV. Cellular Therapies
a. Pluripotent Stem Cells
i. Embryonic Stem Cell and Induced Pluripotent Stem Cell Derivation
ii. Differentiation Potential
iii. Clinical Applications
b. Adult Stem Cells
i. Adult Stem Cells: Hematopoietic, Mesenchymal, Cardiac, Skeletal, and Neural Stem Cells
1. Derivation
2. Differentiation Potential
3. Clinical Applications
V. Cell-Based Therapies
a. Extracellular Vesicles
i. Biogenesis
ii. Collection from Cells
iii. Modes of Action
iv. Therapeutic Applications
VI. Biomaterials and Tissue Engineering
a. Biomaterials in Tissue Engineering
i. Biomaterial Design and Synthesis
ii. 3D Printing of Cellular and Acellular Constructs
b. Biomedical Applications
i. Material Degradation
ii. Tissue Repair
VII. Gene Therapies
a. Strategies of Gene Therapy
i. Ex Vivo vs. In Vivo
b. Gene Transfer Vectors and Targeting Strategies
c. Clinical Applications
VIII. Cell Engineering
a. Techniques for Enhancing Potency
b. Cell Surface Modification
c. Homing Cascade and Improvement
IX. Bioreactors and Cell Manufacturing
a. Cell Expansion Bioreactors
b. Tissue Engineering Bioreactors
c. Organ-on-Chip Systems
X. Devices
a. Near Patient Processing Devices
XI. Diagnostic Approaches
a. MRI, CT, PET
b. High-Resolution Microscopy
XII. The Business of Regenerative Medicine
a. Case Study of a Regenerative Medicine Company
b. FDA Regulations
c. Bringing a Product to Market - Pre-Clinical to Clinical
