Course Description
Regenerative bioscience approaches to tissue repair and regeneration, including stem cell therapies, biomaterials, tissue engineering, gene therapies, and cell manufacturing approaches in regenerative medicine. Students will also learn about how novel devices, technologies, and diagnostic tools can enhance regenerative medicine strategies.
Athena Title
Therapies for Tissue Repair
Prerequisite
BIOL 1108 or BIOL 2108H
Semester Course Offered
Offered spring
Grading System
A - F (Traditional)
Course Objectives
In this course, students will learn about regenerative bioscience approaches to tissue repair and regeneration. Students will learn about endogenous repair processes and why the limitations of endogenous repair processes necessitate the development of regenerative medicine strategies. This course will discuss the derivation, differentiation, and clinical applications of stem cell therapies such as embryonic stem cells, induced pluripotent stem cells, amniotic stem cells, cord blood stem cells, and other multipotent adult stem cell populations. Therapeutic applications of cell-derived therapies such as extracellular vesicles will also be covered. Students will learn about biomaterials and tissue engineering, gene therapy, and bioreactor/cell manufacturing approaches in regenerative medicine. Students will also learn about how novel devices, technologies, and diagnostic tools can be used to enhance regenerative medicine strategies. The business of regenerative medicine will be discussed, including FDA regulations and how to take a product from the pre-clinical to clinical stages.
Topical Outline
I. Overview of Regenerative Bioscience a. Brief History of Regenerative Bioscience b. Mechanisms of Vertebrate Regeneration i. Cellular Regrowth ii. Regeneration from Pre-existing Parent Cells iii. Transdifferentiation iv. Activation of Adult Stem Cells c. Modeling Approaches II. Tissue Injury Responses a. Inflammatory Cells b. Pro- and Anti-inflammatory Cytokines and Chemokines c. Inflammatory Cascade d. Acute and Chronic Inflammation e. Oxidative Stress f. Mechanical Responses III. Endogenous Repair Mechanisms a. Skin b. Neural Tissues c. Musculoskeletal Tissues d. Cardiac Tissue e. Hemopoietic System IV. Cellular Therapies a. Pluripotent Stem Cells i. Embryonic Stem Cell and Induced Pluripotent Stem Cell Derivation ii. Differentiation Potential iii. Clinical Applications b. Adult Stem Cells i. Adult Stem Cells: Hematopoietic, Mesenchymal, Cardiac, Skeletal, and Neural Stem Cells 1. Derivation 2. Differentiation Potential 3. Clinical Applications V. Cell-Based Therapies a. Extracellular Vesicles i. Biogenesis ii. Collection from Cells iii. Modes of Action iv. Therapeutic Applications VI. Biomaterials and Tissue Engineering a. Biomaterials in Tissue Engineering i. Biomaterial Design and Synthesis ii. 3D Printing of Cellular and Acellular Constructs b. Biomedical Applications i. Material Degradation ii. Tissue Repair VII. Gene Therapies a. Strategies of Gene Therapy i. Ex Vivo vs. In Vivo b. Gene Transfer Vectors and Targeting Strategies c. Clinical Applications VIII. Cell Engineering a. Techniques for Enhancing Potency b. Cell Surface Modification c. Homing Cascade and Improvement IX. Bioreactors and Cell Manufacturing a. Cell Expansion Bioreactors b. Tissue Engineering Bioreactors c. Organ-on-Chip Systems X. Devices a. Near Patient Processing Devices XI. Diagnostic Approaches a. MRI, CT, PET b. High-Resolution Microscopy XII. The Business of Regenerative Medicine a. Case Study of a Regenerative Medicine Company b. FDA Regulations c. Bringing a Product to Market- Pre-Clinical to Clinical
Syllabus