Course Description
Methods and applications of molecular biology and gene technology. These will include PCR, gene isolation strategies and various host-plasmid systems, cDNA and genomic cloning, bioinformatics and searching DNA database, genome projects, recombinant protein and stem cell therapeutics, animal cloning and engineering, microarray and medicine, plant biotechnologies (bio-FUEL). Metabolomic and proteomic.
Additional Requirements for Graduate Students:
Graduate students will be required to identify and read
additional papers from the primary and secondary literature on
an assigned topic, and then write a term paper reviewing and
critiquing the literature found.
Athena Title
Intro to Gene Technology
Prerequisite
BIOL 1107 or BIOL 1107E or BIOL 1108 or BIOL 2107H or BIOL 2108H or permission of department
Semester Course Offered
Offered fall
Grading System
A - F (Traditional)
Course Objectives
The course is designed to provide an introduction to the breadth of research techniques and strategies used in modern genetic engineering technology. The course is not designed to give detailed protocols; there is no laboratory component. Instead the objective is to familiarize students with the wide variety of research options currently available in biotechnology, and provide some theoretical background regarding the applicability of these various techniques to specific scientific inquiries.
Topical Outline
The course syllabus is a general plan for the course; deviations announced to the class by the instructor may be necessary. 1) Introduction to macromolecular structures 2) DNA replication and the lactose opeon 3) Basic DNA cloning techniques/strategies 4) Library construction 5) Nucleic acid hybridization strategies 6) The Polymerase Chain reaction 7) Nucleic acid sequencing 8) Genetic mapping /molecularmarkers 9) Genetic fingerprinting 10) Gene expression systems - bacterial, yeast, Plants, animals 11) Structural and Functional Genomics 12) Bioinformatics 13) Proteomics 14) Animal cloning/ stem cells
Syllabus