An intensive laboratory course emphasizing acquisition of skills in common use in research laboratories. Techniques range from solution preparation to isolation and analysis of DNA and protein. Includes proper procedures for keeping a laboratory notebook, laboratory safety, and time management.
Athena Title
Basic Skills in the Laboratory
Prerequisite
[(BIOL 1107 and BIOL 1107L) or (BIOL 2107H and BIOL 2107L)] and [(CHEM 1212 and CHEM 1212L) or (CHEM 1412 and CHEM 1412L)]
Semester Course Offered
Offered fall, spring and summer
Grading System
A - F (Traditional)
Student Learning Outcomes
Students will develop skills to be a resourceful laboratory worker.
Students will learn how to interpret a laboratory protocol and set up an experiment.
Students will become well-practiced in laboratory skills.
Students will be able to critically interpret results.
Students will gain practice communicating results through scientific writing and oral presentation.
Topical Outline
Basic laboratory safety
Pipette and balance usage
Basic laboratory calculations
Culture media preparation
Sterile technique
Culture-based bacterial isolation and quantification
Protein purification, dialysis, and quantification
SDS PAGE
Enzyme kinetics
DNA extraction
PCR
Gel electrophoresis
Experimental design, methods development, and troubleshooting
