Course Description
The accompanying lab section to Avian Virus Diseases. Students will perform common diagnostic tests utilized in the avian diagnostic laboratory such as inoculation of chicken embryos and cell cultures for isolation of viruses, molecular virology techniques, quantification of viruses, and serum neutralization.
Athena Title
Avian Virus Diseases Lab
Non-Traditional Format
Class will convene on Mondays, Tuesdays, Wednesdays, and Fridays.
Pre or Corequisite
Permission of department
Semester Course Offered
Offered fall
Grading System
A - F (Traditional)
Course Objectives
1. To familiarize the student with the current techniques used to isolate and identify avian viruses. 2. To perform all steps involved in preparing and maintaining cell cultures of avian origin. 3. To recognize the effect exerted by important avian viruses in chicken embryo and cell cultures. 4. To perform the different serological tests used to quantitate antibodies against several avian viruses. 5. To manipulate laboratory equipment. 6. To prepare and standardize antigens used in different serologic tests. 7. To understand the basic principles of biotechnology in avian medicine.
Topical Outline
1. Biosafety - lab instructions 2. The HA test=qualitative and quantitative 3. The HI test 4. NDV vaccine titration - effect of lipid solvents 5. Preparation of chicken embryo fibroblast (CEF) 6. Plaque reduction test (PRT) in cell culture 7. Sample preparation 8. Immunodiffusion test 9. Prepare chicken embryo kidney cells (CEKC) in coverslips 10. Infect CEKC (IBV and REO) 11. FA test (IBV) 12. IBV VN in chicken embryos (Alpha test) 13. Prepare CEF in roller bottles and plates 14. Prepare secondary CEF 15. Titrate SB1 vaccine - titrate HVT vaccine 16. Identification of plaques produced by Marek's disease virus 17. " " " " " " 18. " " " " " " 19. " " " " " " 20. LT and Fowl pox embryo inoculation 21. AE embryo susceptibility test - VN test in embryos 22. IC inoculation of day-old chickens (AE) 23. Preparation of chicken embryo liver cells (CELIC) 24. Inoculate CELIC for IUDR test 25. Identification of pocks in CAM's 26. IBDV VN test in microplates 27. Tracheal rings 28. Electrophoresis: demonstration - Stain and read VN test 29. ELISA procedures 30. RFLP and PCR techniques