Course Description
Current techniques in molecular analysis of pathogens. Emphasis is placed on bioinformatics and procedures for genomic analysis, rapid isolation and characterization of parasite molecules, and the detection and identification of parasites in host vector.
Athena Title
ADV MOLECULAR TECH
Equivalent Courses
Not open to students with credit in PARA 8080L
Semester Course Offered
Offered summer semester every even-numbered year.
Grading System
A - F (Traditional)
Course Objectives
The objective of this course is to provide both the theoretical background on state-of-the-art techniques and hands-on laboratory time to acquaint the student with the actual protocol. The course is divided between bioinformatics instruction and exercises, and molecular cell biological techniques.
Topical Outline
Transfection: Transient and stable Selectable markers Expression of Green fluorescent protein, and luciferase Gene disruption by homologous integration Gene isolation/characterization: Homologous PCR:Identifying conserved regions and design of PCR primers Mutation detection: Mutation specific PCR Single strand conformational polymorphism Sequence analysis/bioinformatics: Automated sequencing and assembly of sequences into contigs Relationship of publication to sequence databases Searching online databases and interpreting results Sequence retrieval Accessing and utilizing genome project sequence data Annotation methods for genome sequence projects Multiple sequence alignment Introduction to phylogenetic analysis Analysis of sequence diversity and selective pressure Analysis of protein sequences Expression analysis: RNA isolation Reverse transcription PCR Real-time PCR analysis of gene expression Microarray analysis of gene expression Gene Knockdowns: siRNA Inducible knockdown systems
Syllabus
Public CV