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Introduction to NMR Spectroscopy


Course Description

Principles and instrumental aspects of NMR spectroscopy, including pulsed Fourier transform techniques, proton and carbon-13 techniques used in the analysis of organic compounds, polypeptides and other small molecules. Students also learn to operate NMR spectrometers in the Chemistry Department.

Additional Requirements for Graduate Students:
Graduate students are required to complete supplemental, advanced laboratory exercises and are expected to demonstrate advanced instrumental proficiency.


Athena Title

Introductory NMR


Prerequisite

(CHEM 2211 and CHEM 2212) or (CHEM 2311H or CHEM 2312H) or (CHEM 2411 and CHEM 2412)


Semester Course Offered

Offered fall


Grading System

A - F (Traditional)


Course Objectives

This introductory course is intended to serve a broad audience seeking a background in principles behind nuclear magnetic resonance spectrometry (NMR) and to provide a practical introduction to how data are acquired and analyzed. Material is presented partly in lecture and partly in laboratory formats. Upon successful completion of this course, a student will be able to operate an NMR spectrometer, and to acquire and interpret data for the most basic types of analyses. This course will serve as a prerequisite to more advanced courses offered in biomolecular NMR as well as other application-oriented courses.


Topical Outline

Basic Principles 1H and 13C Chemical Shifts 1H/1H, 13C/1H, 13C/13C Couplings 1D 1H and 13C NMR 1D Double Resonance NMR Complex 1D Pulse Sequences (Spin-Echo, INEPT, DEPT) 2D NMR 2D NMR Pulse Sequences (COSY, TOCSY, HETCOR, HSQC) The Nuclear Overhauser Effect (NOESY)


Syllabus