Course Description
Genome editing technology has exploded since the recent discovery of bacterial CRISPR systems. Our goal is to present the material while new applications are emerging every day and before a textbook is written! Cutting edge knowledge will enable you to shape the future.
Additional Requirements for Graduate Students:
Graduate students will be required to write a mock manuscript review that follows a simple rubric.
Athena Title
Genome Editing
Prerequisite
MIBO 3500 or MIBO 3500E
Semester Course Offered
Offered fall
Grading System
A - F (Traditional)
Course Objectives
Students who complete the course will be able to: 1. describe the basic mechanisms that underlie genome editing technology. 2. describe major ways in which the technology has been applied. 3. assess the connections between reporting in the popular media and scientific foundations. 4. apply genome editing principles to new biomedical problems. 5. identify significant recent research reported in peer-reviewed literature.
Topical Outline
1. Review of molecular biology 2. CRISPR moving parts 3. CRISPR historical origins 4. What happens at a DS break? 5. Off-target effects and sgRNA design 6. Gene regulator CRISPRs 7. Alternate technologies 8. Applications - cell screening 9. Applications - organism engineering 10. Applications - human disease 11. Applications - chromosome imaging 12. Current literature discussion
Syllabus