| Course ID: | BIOL 3110L. 4 hours. 12 hours lab per week. |
| Course Title: | Basic Skills in the Laboratory |
Course
Description: | An intensive laboratory course emphasizing acquisition of skills in common use in research laboratories. Techniques range from solution preparation to isolation and analysis of DNA and protein. Includes proper procedures for keeping a laboratory notebook, laboratory safety, and time management. |
| Oasis Title: | Basic Skills in the Laboratory |
| Prerequisite: | [(BIOL 1107 and BIOL 1107L) or (BIOL 2107H and BIOL 2107L)] and [(CHEM 1212 and CHEM 1212L) or (CHEM 1412 and CHEM 1412L)] |
Semester Course
Offered: | Offered fall, spring and summer semester every year. |
| Grading System: | A-F (Traditional) |
|
| Course Objectives: | • Become familiar with general laboratory practice,
rules, and bio-safety regulations
• Develop technical skill and expertise to perform a
protocol correctly and accurately
• Understand the rationale for steps in a protocol
• Learn how to:
o Interpret and analyze data
o Present data in a clear and meaningful manner
o Maintain a laboratory notebook |
| Topical Outline: | Lab Topics:
I: Laboratory Calculations and Pipetting
II: Preparation of LB Media and Agar
III: Restriction Enzymes and Electrophoresis
IV: Plasmid Purification
V: PCR-based VNTR Human DNA Typing
VI: pGLO Transformation and Protein Purification
(Hydrophobic Interaction Chromotography)
VII: Lab Report Project
Restriction enzyme digestion of the pAMP and pKAN plasmids
Ligation and transformation of recombinant plasmids
Purification and restriction enzyme analysis of recombinant
plasmids
VIII: ELISA (Enzyme-Linked ImmunoSorbant Assay)
IX: Protein Purification and Electrophoresis
X: Western Blot |