| Course ID: | PBIO(CRSS) 4500/6500. 3 hours. |
| Course Title: | Introduction to Gene Technology |
Course
Description: | Methods and applications of molecular biology and gene
technology. These will include PCR, gene isolation strategies
and various host-plasmid systems, cDNA and genomic cloning,
bioinformatics and searching DNA database, genome projects,
recombinant protein and stem cell therapeutics, animal cloning
and engineering, microarray and medicine, plant biotechnologies
(bio-FUEL). Metabolomic and proteomic. |
| Oasis Title: | Intro to Gene Technology |
| Prerequisite: | BIOL 1107 or BIOL 1107E or BIOL 1108 or BIOL 2107H or BIOL 2108H or permission of department |
Semester Course
Offered: | Offered fall semester every year. |
| Grading System: | A-F (Traditional) |
|
| Course Objectives: | The course is designed to provide an introduction to the
breadth of research techniques and strategies used in modern
genetic engineering technology. The course is not designed to
give detailed protocols; there is no laboratory component.
Instead the objective is to familiarize students with the wide
variety of research options currently available in
biotechnology, and provide some theoretical background
regarding the applicability of these various techniques to
specific scientific inquiries. |
| Topical Outline: | The course syllabus is a general plan for the course; deviations announced to the
class by the instructor may be necessary.
1) Introduction to macromolecular structures
2) DNA replication and the lactose opeon
3) Basic DNA cloning techniques/strategies
4) Library construction
5) Nucleic acid hybridization strategies
6) The Polymerase Chain reaction
7) Nucleic acid sequencing
8) Genetic mapping /molecularmarkers
9) Genetic fingerprinting
10) Gene expression systems - bacterial, yeast, Plants, animals
11) Structural and Functional Genomics
12) Bioinformatics
13) Proteomics
14) Animal cloning/ stem cells |
| Honor Code Reference: | All academic work must meet the standards contained in "A Culture of Honesty."
Each student is responsible to inform themselves about those standards before
performing any academic work. |